Advanced in vitro exposure systems.

5. Mar. 2015

The mutagenic assessment of mainstream cigarette smoke using the Ames assay: A multi-strain approach

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

doi:10.1016/j.mrgentox.2015.03.006

Authors
David Thornea, Joanne Kilfordb, Michael Hollingsb, Annette Dalrymplea, Mark Ballantyneb, Clive Mereditha, Deborah Dillona

a British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

 

Wholesmoke Exposure with the VITROCELL® smoking robot VC 10® and the quartz crystal microbalance technology for maintaining the standard Ames Assay

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20. Feb. 2015

Repeated exposure to carbon nanotube-based aerosols does not affect the functional properties of a 3D human epithelial airway model

Nanotoxicology, Early Online: 1–11 (doi: 10.3109/17435390.2014.993344)

Authors
Savvina Chortarea1, Martin J. D. Clift1, Dimitri Vanhecke1, Carola Endes1, Peter Wick2, Alke Petri-Fink1, and Barbara Rothen-Rutishauser1
1Department of BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Marly, Switzerland and
2Laboratory for Materials-Biology Interactions, EMPA, Swiss Federal Laboratories for Materials, Science and Technology, St. Gallen, Switzerland

Exposition of Carbone Nanotubes on 3D lung models in the air-liquid interface exposure system to measure oxydative stress and inflammatory response.

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12. Feb. 2015

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

New video protocol Exposure of 3D cell cultures using VITROCELL® 24/48 system

jove, 12 Februray 2015, Issue 96 (doi: 10.3791/52325)

Authors
Diana Kuehn1, Shoaib Majeed1, Emmanuel Guedj1, Remi Dulize1, Karine Baumer1, Anita Iskandar1, Stephanie Boue1, Florian Martin1, Radina Kostadinova1, Carole Mathis1, Nikolai V. Ivanov1, Stefan Frentzel1, Julia Hoeng1, Manuel C. Peitsch1

1 Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A

The aim of this video protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke at the air-liquid interface to measure ciliary beating and TEER.

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12. Nov. 2014

Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke

Chemistry Central Journal 2014, 8:62

doi:10.1186/s13065-014-0062-3

Authors
Shoaib Majeed1, Stefan Frentzel1, Sandra Wagner2, Diana Kuehn1, Patrice Leroy1, Philippe A. Guy1, Arno Knorr1, Julia Hoeng1 and Manuel C. Peitsch1

1 Philip Morris Research and Development, Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
2 Eurofins Umwelt West GmbH, Vorgebirgsstraße20, D-50389 Wesseling, Germany

 

The connection of a VITROCELL® 24/48 system to a 30-port carousel smoking machine is shown. The exposure is to verify the cigarette smoke aerosol distribution, the utility of VITROCELL® crystal quartz microbalances and the amount of particles deposited per surface area. The results are shown in cell viability and WST-1 oxidation.

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30. Oct. 2014

Comparative In Vitro Toxicity Profile of Electronic and Tobacco Cigarettes, Smokeless Tobacco and Nicotine Replacement Therapy Products: E-Liquids, Extracts and Collected Aerosols

International Journal of Environmental Research and Public Health 2014

11325-11347; doi:10.3390/ijerph111111325

Authors
Manoj Misra, Robert D. Leverette, Bethany T. Cooper, Melanee B. Bennett and Steven E. Brown

Lorillard Tobacco Company, A.W. Spears Research Center, 420 North English Street, Greensboro, North Carolina 27405, USA

 

The in vitro exposure of tobacco and e-cigarettes that were smoked on a VITROCELL VC 10® smoking robot. The Results are shown with the NRU, IL-8, Ames and Micronucleus assay.

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30. Oct. 2014

An inter-machine comparison of tobacco smoke particle deposition in vitro from six independent smoke exposure systems

Toxicology in Vitro

Volume 28, Issue 7, October 2014, Pages 1320-1328 (doi: 10.1016/j.tiv.2014.06.012)

Authors
J. Adamsona, D. Thornea, G. Erringtona, W. Fieldsb, X. Lic, , R. Payned, T. Krebse, A. Dalrymplea, K. Fowlerb, D. Dillona, F. Xiec, , C. Mereditha

a British American Tobacco, Group R&D, Southampton SO15 8TL, UK
b R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA
c Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,
No.2 Fengyang Street, High-Tech Zone, Zhengzhou, PR China
d Covance Laboratories Ltd., Otley Road, Harrogate HG3 1PY, UK
e Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

 

Comparison of different smoke exposure systems to demonstrate VC 10® suitability for tobacco smoke exposure in vitro. The results are shown in the particle deposition across the modules.

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8. Oct. 2014

The Response of Human Nasal and Bronchial Organotypic Tissue Cultures to Repeated Whole Cigarette Smoke Exposure

International Journal of Toxicology (October 8, 2014)

Authors
Marja Talikka1, Radina Kostadinova1, Yang Xiang1, Carole Mathis1, Alain Sewer1, Shoaib Majeed1, Diana Kuehn1, Stefan Frentzel1, Celine Merg1, Marcel Geertz1, Florian Martin1, Nikolai V. Ivanov1, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

 

Whole Smoke Exposure on nasal and bronchial cells using a VITROCELL®24 system in comparison to animal testings.

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23. Sep. 2014

An in vitro testing strategy towards mimicking the inhalation of high aspect ratio nanoparticles

High aspect ratio nanoparticles (HARN) were analyzed at the air/liquid interface using VITROCELL Cloud technology.

Particle and Fibre Toxicology 2014, 11:40 (doi:10.1186/s12989-014-0040-x)

Authors
Carola Endes1, Otmar Schmid2, Calum Kinnear1, Silvana Mueller3, Sandra Camarero-Espinosa3, Dimitri Vanhecke1, E. Johan Foster3, Alke Petri-Fink1,4, Barbara Rothen-Rutishauser1, Christoph Weder3 and Martin J.D. Clift.

1 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland
2 Comprehensive Pneumology Centre, Institute of Lung Biology and Disease, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
3 Polymer Chemistry and Materials, Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland
4 Department of Chemistry, University of Fribourg, Fribourg, Switzerland

 

High aspect ratio nanoparticles (HARN) were analyzed at the air-liquid interface using the VITROCELL® Cloud technology. The successful approach towards investigation of the hazard of HARN aerosols in vitro are demonstrated by measured cytotoxicity, oxidative stress and inflammatory responses. 

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19. Sep. 2014

Silica nanoparticles are less toxic to human lung cells when deposited at the air-liquid interface compared to conventional submerged exposure

Beilstein Journal of Nanotechnology

2014, 5, 1590–1602 (doi:10.3762/bjnano.5.171)

Authors
Alicja Panas1, Andreas Comouth2, Harald Saathoff2, Thomas Leisner2, Marco Al-Rawi1, Michael Simon3, Gunnar Seemann3, Olaf Dössel3, Sonja Mülhopt4, Hanns-Rudolf Paur4, Susanne Fritsch-Decker1, Carsten Weiss1and Silvia Diabaté1

1 Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus North, Hermann- von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
2 Institute for Meteorology and Climate Research, Karlsruhe Institute of Technology, Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
3 Institute of Biomedical Engineering, Karlsruhe Institute of Technology, Campus South, Kaiserstraße 12, 76128 Karlsruhe, Germany
4 Institute for Technical Chemistry, Karlsruhe Institute of Technology, Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany

Exposition of humidified nanoparticles on A549 lung epithelia cells using the air-liquid interface. The experiments shows less cytotoxic and inflammatory responses of the cells to silica nanoparticles.

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21. Jul. 2014

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

informa healthcare

informa healthcare, posted online on July 21, 2014. (doi:10.3109/15376516.2014.943441)

Authors
Walter K. Schlage*, Anita R. Iskandar*, Radina Kostadinova*, Yang Xiang*, Alain Sewer*, Shoaib Majeed, Diana Kuehn, Stefan Frentzel, Marja Talikka, Marcel Geertz, Carole Mathis, Nikolai Ivanov, Julia Hoeng, and Manuel C. Peitsch

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

*Equal contribution

Exposition of cigarette smoke to tissue cultures at the air–liquid interface shows in combination of the microarray technology, gene-set analysis and a novel computational modeling an increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity. The usefulness of in vitro exposure to buccal and gingival organotypic epithelial tissue cultures for the impact assessment of cigarette smoke is demonstrated. 

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