https://doi.org/10.1186/s12989-019-0297-1

Ionel Fizeșan¹, Sébastien Cambier², Elisa Moschini², Aline Chary², Inge Nelissen³, Johanna Ziebel², Jean-Nicolas Audinot⁴, Tom Wirtz⁴, Marcin Kruszewski^5,6, Anca Pop¹, Béla Kiss¹, Tommaso Serchi², Felicia Loghin¹ and Arno C. Gutleb²

¹Toxicology Department, Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. 
²Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg. 
³Health Unit, Flemish Institute for Technological Research (VITO NV), Mol, Belgium. 
⁴Material Research and Technology (MRT) Department, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg. 
⁵Faculty of Medicine, University of Information Technology and Management in Rzeszow, Sucharskiego 2, Rzeszow, Poland. 
⁶Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, Warszawa, Poland

This study aimed to investigate the distinctive biological effects elicited by two different sizes of spherical silver particles (Ag20 and Ag200) and of PVP-coated silver nanowires (AgNWs) in a 3D tetra-culture in vitro model, representative for the alveolar barrier using the Vitrocell™Cloud System. The second objective was to investigate the influence of the AgNMs shape on biological responses. Only a few scientific articles studied the biological effects of AgNWs, and to the best of our knowledge, none investigated the biological effects using an ALI system.

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Anita R. Iskandar, Filippo Zanetti, Athanasios Kondylis, Florian Martin, Alain Sewer, Laura Ortega Torres, Shoaib Majeed, Sandro Steiner, Emmanuel Guedj, Celine Merg, Thomas Schneider, Keyur Trivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

The impacts of an acute exposure to cigarette smoke (CS) and to aerosol from a novel electronic cigarette (EC) device using MESH™ technology were assessed using human organotypic buccal epithelial cultures and small airway epithelial cultures. A paired design was implemented: in parallel to the exposure to CS or EC aerosol, cultures were also exposed to air in the same exposure module. Tissue damage was not seen in cultures exposed to the IQOS MESH™ Classic Tobacco aerosol despite resulting in greater concentrations of deposited nicotine. In buccal cultures, CS and IQOS MESH™ Classic Tobacco aerosol elicited different infammatory response.

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DOI:10.1007/s11739-019-02055-x
Filippo Zanetti¹, Athanasios Kondylis¹, Florian Martin¹, Patrice Leroy¹, Shoaib Majeed¹, Sandro Steiner¹, Yang Xiang¹, Laura Ortega Torres¹, Keyur Trivedi¹, Emmanuel Guedj¹, Celine Merg¹, Stefan Frentzel1, Nikolai V. Ivanov¹, Utkarsh Doshi², Kyeonghee Monica Lee², Willie J. McKinneyJr², Manuel C. Peitsch¹, Julia Hoeng¹

¹ Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
² Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

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DOI: 10.1016/j.yrtph.2019.01.036

Czekala L¹, Simms L², Stevenson M², Tschierske N², Maione AG³, Walele T².
¹ Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK. Electronic address: Lukasz.Czekala@uk.imptob.com.
² Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK.
³ MatTek, 200 Homer Ave., Ashland, MA, USA.

In this research article a respiratory epithelial model, EpiAirway, was used to examine the biological effects of nicotine-containing blu PLUS + e-cigarettes, in comparison to conventional cigarette smoke. Tissues were exposed at the air-liquid interface to cigarette smoke or e-cigarette aerosol generated using a VITROCELL VC1 smoking/vaping robot. 

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https://doi.org/10.1016/j.tiv.2018.12.010

Brian M. Keyser^a, Robert Leverette^a, Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a
a RAI Services Company1, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
^b Covance Laboratories Ltd., North Yorkshire, UK

The aims of this study were to investigate dosimetry during whole smoke exposure in the VITROCELL® VC10® smoking robot using QCMs and to determine whether photometers could be used for concurrent assessment of dose during in vitro whole smoke exposures.

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