Lukasz Czekala¹, Matthew Stevenson¹, Liam Simms¹, Nicole Tschierske¹, Anna G. Maione², Tanvir Walele^3
1 Imperial Tobacco Ltd, 121 Winterstoke Road, Bristol, BS3 2LL UK
² MatTek, 200 Homer Ave., Ashland, MA, USA
³ Fontem Ventures B.V., an Imperial Brands PLC Company, Radarweg60, 1043 NT Amsterdam

A 3D in vitro organotypic model of the human respiratory epithelium was cultured at the Air-Liquid Interface (ALI) and exposed to cigarette smoke and e-cigarette aerosol, generated by using a VITROCELL® VC1 smoking machine under Health Canada Intense and CORESTA conditions.
The results are shown in tissue viability (MTT), barrier integrity (TEER), Barrier function, tissue secretion of the pro-inflammatory cytokines (IL-6 and IL-8). The presence of 8-isoprostane is used as an indicator of oxidative stress and antioxidant deficiency. Additionally the tissue morphology was assessed by H&E staining and immunofluorescent staining was conducted for specific markers.

Read more

Filippo Zanetti¹, Xiaoyi Zhao^2,3, Shoaib Majeed¹, Hans Malmstrom², Manuel C. Peitsch¹, Yanfang Ren², Julia Hoeng¹

¹ PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland (part of Philip Morris International group of companies)
² University of Rochester Eastman Institute for Oral Health, Rochester, New York
³ Peking University School of Stomatology, Peking, China

In this study, the effects of 3R4F cigarette smoke and THS2.2 aerosol on color stability and surface roughness of premolar human teeth were investigated.

The poster was presented at SRNT 2018, https://www.srnt.org/

Read more

https://doi.org/10.1016/j.tiv.2018.01.010
E. Bishop, L. Haswell, J. Adamson, S. Costigan, D. Thorne, M. Gaca
British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK

A reconstituted 3D human airway epithelium was exposed to undiluted aerosols at the air-liquid interface, using a Vitrocell VC 10 under ISO and HCI conditions. A 3R4F reference cigarette and a commercially available e-cigarette product were tested on their effect on CBF and TEER.

Read more

T. Schneider, A. Elamin, A.R. Iskandar, F. Zanetti, C. Merg, A. Sewer, B. Titz, P. Leroy, E. Guedj, S. Majeed, L. Ortega-Torres, W. Schlage, C. Mathis, F. Martin, S. Frentzel, N. V. Ivanov, M.C. Peitsch, J. Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland (Part of Philip Morris International group of companies)

The objectives of this study included to demonstrate robustness and reproducibility of systems biology data obtained from an organotypic in vitro nasal culture model for cigarette smoke and aerosol exposure assessment. Cellular stress and pro-inflammatory responses were investigated on the primary nasal epithelial cells of a non-smoker.

Read more

Filippo Zanetti, Alain Sewer, Patrice Leroy, Shoaib Majeed, Laura Ortega Torres, Céline Merg, Maica Corciulo, Keyur Trivedi, Emmanuel Guedj, Ashraf Elamin, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
Philip Morris International Research and Development, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

The objective of this study was to assess and compare the effects of repeated exposures to the aerosol of CHTP1.2 with cigarette smoke generated from reference cigarettes (3R4F) on human gingival organotypic epithelial cultures. The results were evaluated in using Adenylate Kinase (AK)-based cytotoxicity assay, histological analysis, pro-inflammatory mediators and Microarray data processing and analysis.

The poster was presented at Eurotox 2017, http://www.eurotox2017.com

Read more