DOI:10.1038/s41598-017-11038-x
Juliette Sailland¹, Astrid Grosche^1,3, Nathalie Baumlin¹, John S. Dennis¹, Andreas Schmid¹, Stefanie Krick^1,2 & Matthias Salathe^1
1 Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
² Division of Pulmonary, Allergy & Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
³ Department of Radiation Oncology, University of Florida, Gainesville, FL, 32610, USA.

The aim of the study is to examine the effects of cigarette smoke on voltage dependent K+ channels, cystic fibrosis transmembrane conductance regulator activity and airway surface liquid volume on primary human bronchial epithelial cells from non-smokers and smokers, cultured at the air-liquid interface to full differentiation.

Read more

A. Chary ^1,2, T. Serchi¹, J. Hennen², B. Bloemeke², J. Ezendam³, S. Cambier¹, E. Moschini¹, A.C. Gutleb^1
1Department of Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg,
²Department of Environmental Toxicology, University of Trier, Trier, Germany,
³RIVM Centre for Environmental Protection (GZB), National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands.

The aim of this study is to adapt the 3D-co-culture system previously developed by LIST (Klein et al.1,2) to allow the in vitro study of respiratory sensitization processes and to
find markers for the assessment of the respiratory sensitizing potential of inhaled compounds and mimicking the alveolar barrier to study cell-to-cell communication and inflammatory effects of nano particles at the air-liquid-interface.

Read more

DOI:10.1016/j.etap.2017.06.023 

Shimin Zhang^a,b, Xiang Li^a, Fuwei Xie^a, Kejian Liu^a, Huimin Liu^a, Jianping Xie^a

^a Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China
^b Technique Center of Tobacco Production, PingDingshanTobacco Company of Henan Tobacco Monopoly Bureau, PingDingshan 467000, China

The purpose of this study is to evaluate oxidative stress induced by whole cigarette smoke based on the air-liquid interface exposure system. The cytotoxicity of whole smoke were measured by MTT and NRU assays, and several intracellular and extracellular oxidative stress biomarkers such as GSH and GSSG, MDA, HNE, EC-SOD and 8-OHdG were investigated in A549 and BEAS–2B cells.

Read more

S. Constant¹, A. Stelzl^2,3, O. Eickelberg^2,3,4, O. Schmid^2,3
1Epithelix, Genève, Switzerland
²Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany
³Institute of Lung Biology and Disease, Helmholtz Zentrum München, Munich/Neuherberg, Germany
⁴University Hospital of the Ludwig Maximilians University (LMU), Munich, Germany

The poster shows the ALICE-Cloud technology combined with primary ALI 3D cell cultures. The effect of nebulized e-liquids were investigated in terms of tissue integrity, celia beating frequency, mucin secretion and IL-8 secretion.

Read more

DOI: 10.1021/acsnano.7b01992

Savvina Chortarea,¹ Hana Barosova,¹ Martin James David Clift,² Peter Wick,³ Alke Petri-Fink,^1,4 and Barbara Rothen-Rutishauser,¹

¹BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, CH-1700 Fribourg, Switzerland
²In Vitro Toxicology Group, Swansea University Medical School, Swansea SA2 8PP, Wales, U.K.
³Laboratory for Materials-Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science and Technology, 9014 St Gallen, Switzerland
⁴Department of Chemistry, University of Fribourg, CH-1700 Fribourg, Switzerland

The aim of this study was to realistically mimic subchronic inhalation of multiwalled carbone nanotubes in vitro, using the air−liquid interface cell exposure system for aerosol exposures on reconstituted human bronchial tissue from healthy and asthmatic donors. Cytotoxicity, cell morphology, cilia beating frequency, mucociliary clearance, gene expression analysis of oxidative stress and (pro‑)inflammatory markers as well as (pro‑)inflammatory cytokine secretion were successfully observed.

Read more