Advanced in vitro exposure systems.

29. Sep. 2015

Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure

DOI 10.1186/s12989-015-0104-6

Linda C. Stoehr1,2†, Carola Endes3†, Isabella Radauer-Preiml1, Matthew S. P. Boyles1, Eudald Casals4, Sandor Balog5, Markus Pesch2, Alke Petri-Fink3, Barbara Rothen-Rutishauser3, Martin Himly1, Martin J. D. Clift3 and Albert Duschl1*

1Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria.

2Grimm Aerosol Technik GmbH & Co. KG, Ainring, Germany.
3BioNanomaterials, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
4Institut Català de Nanotecnologia (ICN), Bellaterra, Spain.
5Soft Matter Scattering, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
* Correspondence: albert.duschl@sbg.ac.at


The use of fluorescence-based reporter cell lines can provide a useful tool in screening the pro-inflammatory response following NP exposure in the air-liquid interface. The results are shown in the IL-8 assay, qRT-PCR ans ELISA.

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23. Sep. 2015

Toxicological validation during the development of new catalytic systems using air / liquid interface cell exposure system

EUROTOX 13.-16. September 2015

Authors

Y. Landkocz1, M. Al Zallouha1, J. Brunet2, R. Cousin2, J.M. Halket3, E. Genty2, D. Courcot1, S. Siffert2, P. Shirali1, S. Billet1

(1) UCEIV, Chimie et Toxicologie des Emissions Atmosphériques, EA4492, Dunkerque, France; Sylvain.Billet@univ-littoral.fr
(2) UCEIV, Traitement Catalytique et Energie Propre, EA4492, Dunkerque, France
(3) Mass Spectrometry Facility, King’s College, London, United Kingdom

 

The aim of this study was to determine the most efficient catalyst for toluene oxidation. Thereby A549 lung cells were exposed using an air/liquid interface (ALI) system and the results are performed with the LDH cytotoxicity test and XME gene expression measurement.

The suitability of using Vitrocell® system as an innovative, direct and dynamic model of ALI exposure in the development of new catalysts is successfully shown.

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23. Sep. 2015

Airway epithelium co-cultured with immune cells for a better assessment of the low dose effects of environmental pollutants

EUROTOX 13.-16. September 2015

Authors

Emilie RICQUEBOURG, Gabrielle VERNOUILLET, Isabelle MOMAS, Nathalie SETA, Sophie ACHARD

Public Health and Environment Laboratory, EA4064 - Paris Descartes University


Exposure of airborne pollutants to a 3D airway epithelial cell modell in the air-liquid interface to measure the epithelial thickness and the inflammatory respone with the IL-8 assay.

Winner of the price "AstraZeneca Young Investigator Prize for Innovation in Drug Discovery and Development"

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23. Sep. 2015

A comparative assessment of cigarette smoke aerosols using an in vitro air-liquid interface cytotoxicity test.

DOI:10.3109/08958378.2015.1080773

Authors

Thorne D, Dalrymple A, Dillon D, Duke M, Meredith C

British American Tobacco, Group R&D, Southampton, Hampshire, UK.

 

Evaluation of a modified air-liquid interface BALB/c 3T3 cytotoxicity method for the assessment of smoke aerosols in vitro. 

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12. Jul. 2015

Development of an in vitro cytotoxicity model for aerosol exposure using 3D reconstructed human airway tissue; application for assessment of e-cigarette aerosol

DOI:10.1016/j.tiv.2015.05.018

Authors

Louise Neilsona, Courtney Mankusb, David Thornea, George Jacksonb, Jason DeBayb, Clive Mereditha

a British American Tobacco, Group Research and Development, Regents Park Road, Southampton, Hampshire SO15 8TL, United Kingdom
b MatTek Corporation, 200 Homer Avenue, Ashland, MA 01721, United States

 

The presentation of a new highly differentiated 3D airway culture, grown and exposed in the VITROCELL® system, shows an successful compatibility of the tissue and the VITROCELL® system for testing e-cigarettes. The results are shown with the MTT assay for cell viability and measurement of TEER.

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27. Jun. 2015

Translocation of gold nanoparticles across the lung epithelial tissue barrier: Combining in vitro and in silico methods to substitute in vivo experiments

DOI 10.1186/s12989-015-0090-8

Gerald Bachler1,2, Sabrina Losert1,3, Yuki Umehara2, Natalie von Goetz1*, Laura Rodriguez-Lorenzo2, Alke Petri-Fink2,
Barbara Rothen-Rutishauser2 and Konrad Hungerbuehler1

1ETH Zürich, Institute for Chemical and Bioengineering, 8093 Zürich, Switzerland.
2University of Fribourg, Adolphe Merkle Institute, 1700 Fribourg, Switzerland.
3EMPA, Swiss Federal Laboratories for Material Science and Technology, 8600 Dübendorf, Switzerland.

* Correspondence: natalie.von.goetz@chem.ethz.ch

 

This replacement study for animal testings shows aerosolized nanoparticles tested on monolayer epithelial cultures in the air-liquid interface to determine their translocation kinetics across the epithelial tissue barrier.

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3. Jun. 2015

Particulate Matter from Both Heavy Fuel Oil and Diesel Fuel Shipping Emissions Show Strong Biological Effects on Human Lung Cells at Realistic and Comparable In Vitro Exposure Conditions

DOI: 10.1371/journal.pone.0126536

Authors

Sebastian Oeder , Tamara Kanashova , Olli Sippula , Sean C. Sapcariu , Thorsten Streibel , Jose Manuel Arteaga-Salas , Johannes Passig , Marco Dilger, Hanns-Rudolf Paur, Christoph Schlager, Sonja Mülhopt, Silvia Diabaté, Carsten Weiss, Benjamin Stengel, Rom Rabe, Horst Harndorf, Tiina Torvela, Jorma K. Jokiniemi, Maija-Riitta Hirvonen, Carsten Schmidt-Weber, Claudia Traidl-Hoffmann, Kelly A. BéruBé, Anna J. Wlodarczyk, Zoë Prytherch, Bernhard Michalke, Tobias Krebs, André S. H. Prévôt, Michael Kelbg, Josef Tiggesbäumker, Erwin Karg, Gert Jakobi, Sorana Scholtes, Jürgen Schnelle-Kreis, Jutta Lintelmann, Georg Matuschek, Martin Sklorz, Sophie Klingbeil, Jürgen Orasche, Patrick Richthammer, Laarnie Müller, Michael Elsasser, Ahmed Reda, Thomas Gröger, Benedikt Weggler, Theo Schwemer, Hendryk Czech, Christopher P. Rüger, Gülcin Abbaszade, Christian Radischat, Karsten Hiller, Jeroen T. M. Buters , Gunnar Dittmar , Ralf Zimmermann
Institutes

 

Exposition of Heavy Fuel Oil and Diesel Fuel Shipping Emissions on human lung cells in the air-liquid interface exposure system for advanced chemical analysis combined with transcriptional, proteomic and metabolomic profiling and isotope labelling methods.

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22. May. 2015

Testing particle delivery of e-cigarettes

VITROCELL® Application Note for VC 10® S-TYPE Smoking Machine and Particle Photometers

The new application note shows a successfull monitoring of the particle delivery of different e-cigarettes on the setup of a VITROCELL VC 10® S-TYPE Smoking Machine combined with the VITROCELL® Particle Photometer and Monitor Software.

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22. May. 2015

Testing of conventional and e-cigarettes: using EpiAirway and EpiAlveolar with the VITROCELL® in vitro Exposure System

VITROCELL® VC1 Smoking Machine and 12/6 CF Exposure Module at the Air/Liquid Interface.

New MatTek application note shows a state-of-the-art in vitro set up by VITROCELL® Systems for testing combustion cigarettes as well as electronic cigarettes with the endpoints toxicity, oxidative stress, TEER, beating cilia, gene expression and mucus secretion.

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21. Mar. 2015

Automatisiertes Expositionssystem für Zellkulturen an der Gas-Flüssigkeits-Grenzschicht

Feinstaub in der Lunge

(Automated exposure system for cell cultures at the air/liquid interface / article in German)

Authors
Sonja Mülhopt, Tobias Krebs, Dr. Silvia Diabaté, Christoph Schlager, Dr. Hanns-Rudolf Paur

 

Zur Analyse von gasgetragenen Nano- und Feinstpartikel werden Bioassays mit menschlichen Lungenzellkulturen durchgeführt. Mit dem automatisierten Vitrocell-Expositionssystem können Aerosole reproduzierbar auf die Zellen aufgebracht werden.

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