Advanced in vitro exposure systems.

31. Oct. 2016

Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

doi: 10.1155/2016/9461289
Patrick Geraghty,1,2 Nathalie Baumlin,3 Matthias A. Salathe,3 Robert F. Foronjy,1,2 and Jeanine M. D’Armiento4
1Division of Pulmonary & Critical Care Medicine, Department of Medicine, State University of New York Downstate Medical Center, Brooklyn, NY, USA
2Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY, USA
3Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Miami, Miami, FL, USA
4Center for Pulmonary Disease, Department of Anesthesiology, College of Physicians and Surgeons,Columbia University, New York, NY, USA

 

The Expression of ER stress markers after exposure to cigarette smoke was investigated in fully differentiated normal human bronchial epithelial (NHBE) cells isolated from nonsmoking, smoking, and COPD donors and redifferentiated at the air liquid interface.

 

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28. Oct. 2016

Evaluation of an in vitro screening model to assess phosgene inhalation injury

DOI: 10.1080/15376516.2016.1243183

Dorian S. Olivera, Heidi Hoard-Fruchey & Alfred M. Sciuto
Analytical Toxicology Devision, US Army, Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, USA

In vitro studies have clearly shown that phosgene exposure can have overwhelming effects on cellular homeostasis with regard to mediator release, activation of inflammatory pathways and compromised lung function.
In this study an in vitro exposure model with which to evaluate potential therapeutics for edemagenic gas inhalation exposure was tested.
Human bronchial epithelial (16HBE) cell cultures were exposed in the Vitrocell System to phosgene.
Transepithelial electrical resistance (TEER), Cellular viability assay (XTT assay), [14C]-Glutamine oxidation assay, Glucose metabolism assays and Lactate production measurements were taken.

 

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27. Oct. 2016

Usefulness of toxicological validation of VOCs catalytic degradation by airliquid interface exposure system

doi: 10.1016/j.envres.2016.10.027.

Margueritta Al Zallouha, Yann Landkocz, Julien Brunet, Renaud Cousin, Eric Genty, Dominique Courcot, Stéphane Siffert, Pirouz Shirali, Sylvain Billet
Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d′Opale, 189 A Avenue Maurice Schumann, 59140 Dunkerque, France


The exposure of A549 cells to VOCs and by-products at the Air-Liquid Interface exposure system were tested and showed toxic effects. Additionally a catalyst setup coupled to the Vitrocell system for testing by-products of toluene oxidation has been validated. The toxicological analysis is done with a cell viability test (LDH) and a TaqMan gene expression kit (RT-qPCR).

 

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9. Oct. 2016

The mutagenic assessment of electronic-cigarettes and tobacco smoke using the Ames assay in strains TA98 and TA100

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany
Poster No:STPOST34

 

Thorne, D1., Crooks, I1., Hollings, M2., Seymour, A2., Meredith, C1., Gaa, M1.

1 British American Tobacco, R&D Centre, Southampton, SO15 8TL, UK

2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK.

 

The Aim of this study was to assess the mutagenicity of an e-cigarette aerosol, compared to cigarette smoke in tester strains TA98 and TA100 using two different exposure matrices, TPM/eTPM (or ACM – aerosol collected matter) and whole aerosol. A Vitrocell® VC 10 Smoking Robot was used to generate aerosol streams from a traditional reference cigarette (3R4F) and e-cigarette (Vype® ePen). 

 

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9. Oct. 2016

5 out of 7 Tobacco Companies have chosen VITROCELL® for research on combustion and e-cigarettes: A review of aerosol exposure systems relative to the analysis of cytotoxicity

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany

Poster No:STPOST26

 

David Thorne1, Roman Wieczorek2,Toshiro Fukushima3, Han-Jae Shin4, Robert Leverette5, Mark Ballantyne6, Xiang Li 7, Betsy Bombick5

1 British American Tobacco, Group R&D, Southampton, Hampshire, SO15 8TL, UK;
2 Imperial Brands PLC Company, Reemtsma Cigarettenfabriken GmbH, Albert-Einstein-Ring 7, 22761 Hamburg, Germany;
3 Japan Tobacco Inc, Scientific Product Assessment Centre, 6-2 Umegaoka, Aoba-ku Yokohama, Kanagawa 227-8512, Japan;
4 Korean Tobacco & Ginseng Corporation, 30 Gajeong-ro, Yuseong-gu, Daejeon, 305-805, Republic of Korea;
5 RAI Services Company, 401North Main Street, Winston-Salem, NC 27101, USA;
6 Covance Laboratories Ltd, Otley Road, Harrogate HG3 1PY, UK;
7 Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, No.2 Fengyang Street, High-tech Zone, Zhengzhou, PR China

 

Cytotoxicity data (NRU and MTT) from Kentucky reference 3R4F cigarette smoke were assessed on Balb/C 3T3, CHO-K1, A549, Beas-2B, NCI-H292 and HepG2 cell lines on the Vitrocell Exposure System.

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9. Sep. 2016

21st Century Toxicology & In Vitro-Based Testing Methodologies

Julia Hoeng, Anita Iskandar, Filippo Zanetti

Philip Morris International


The studies used human cells grown in three-dimensional culture systems. The cultures were grown on top of an artificial membrane at the air-liquid interface, allowing them to develop ‘organotypic’ tissue complexity. Testcigarette is the Tobacco Heating System 2.2 (THS), a new technology that heats tobacco without burning it. The endpoints are shown in measurements of cytotoxicity, alterations in tissue morphology (histology), impact on processes involved in the metabolism of toxicants (xenobiotic metabolism response), secretion of pro-inflammatory mediators, and perturbation of genome-wide gene profiles. The nasal study also looked at ciliary function.



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8. Sep. 2016

A lab scale measurement technique for the air-liquid interface exposure of human lung cell cultures towards particulate emissions from combustion processes

11th International Particle Toxicology Conference, Sep 26 – 30, 2016 in Singapore


1S. Mülhopt, 3M. Berger, 1C. Schlager, 2M. Dilger, 2S. Diabaté, 3T. Krebs, 4,5Ralf Zimmermann, 4Jeroen Buters, 4Sebastian Oeder, 2C. Weiss, 1H.-R. Paur

1Institute for Technical Chemistry
2Institute of Toxicology and Genetics

3Vitrocell Systems GmbH, Germany
4Helmholtz CentreMunich, Germany
5University of Rostock, Germany
 

The Karlsruhe Exposure System is a lab scale measurement system for the air-liquid interface exposure of human lung cell cultures towards air borne nanoparticles under well defined conditions.

 

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1. Sep. 2016

Aerosol Flow in the Vitrocell 24/48 Exposure System: Flow Mixing and Aerosol Coalescence

Applied In Vitro Toxicology. September 2016, Vol. 2, No. 3: 165-174 

Arkadiusz K. Kuczaj,1,2 Markus Nordlund,1 Santhosh Jayaraju,3 Ed Komen,3 Tobias Krebs,4 Manuel C. Peitsch,1 and Julia Hoeng1
1Philip Morris International Research & Development, Philip Morris Products S.A., Neuchâtel, Switzerland.
2Multiscale Modeling & Simulation, Department of Applied Mathematics, University of Twente, Enschede, The Netherlands.
3NRG, Petten, The Netherlands.
4Vitrocell Systems GmbH, Waldkirch, Germany.
 

In this work, the flow-mixing efficiency of the dilution unit in the Vitrocell® 24/48 (VC24/48) exposure system and the role of thermal coalescence is presented . The dilution unit of the VC24/48 system guarantees efficient and localized mixing/dilution of the aerosol and the thermal coalescence effects do not influence the aerosol droplet size distribution.

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14. Aug. 2016

Copper oxide nanoparticles: Impact on alveolar epithelial-like cells following air-liquid interface exposure

EEMGS Annual Meeting: 14 – 18 August 2016, Copenhagen, Denmark

doi:10.1007/s00204-015-1621-7

Matthias Hufnagel, Sarah Schoch, Bettina Fischer, Andrea Hartwig
Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Food Chemistry and Toxicology

This is work is part of ProCycle (No. 03XP0009), a project funded by the German Federal Ministry of Education and Research.and shows the exposition of copper oxide (CuO) nanoparticles on A549 cells at different doses in the VITROCELL® Cloud, equipped with a quartz crystal microbalance. The effects were determined using the colony formation assay (CFA) and high-throughput RT-qPCR gene expression analyses.

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12. Jul. 2016

Systems Toxicology Assessment of the Biological Impact of a Candidate Modified Risk Tobacco Product on Human Organotypic Oral Epithelial Cultures

DOI:10.1021/acs.chemrestox.6b00174

Chem. Res. Toxicol.2016, 29, 1252−1269

Filippo  Zanetti, Alain  Sewer, Carole  Mathis, Anita  R.  Iskandar, Radina  Kostadinova, Walter K. Schlage, Patrice Leroy, Shoaib Majeed, Emmanuel Guedj, Keyur Trivedi, Florian Martin, Ashraf  Elamin, Celine  Merg, Nikolai  V.  Ivanov, Stefan  Frentzel, Manuel  C.  Peitsch, and  Julia  Hoeng*,

Philip Morris International Research and Development, Quai Jeanrenaud 5, 2000 Neuchatel, Switzerland
Biology Consultant, Max-Baermann-Str. 21, 51429 Bergisch Gladbach, Germany

 
The tobacco heating system (THS) 2.2 and the 3R4F reference cigarette smoke were exposed to human organotypic oral epithelial tissue cultures (EpiOral, MatTek)at the Vitrocell 24/48 exposure system. The results are shown by the evaluation of cytotoxicity and cytochrome P450 activity assays, mRNA and miRNA purification, measurements of secreted proin flammatory markers and histopathological analysis.

 

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