Advanced in vitro exposure systems.

12. Apr. 2018

Development, qualification, validation and application of the Ames test using a VITROCELL® VC10® smoke exposure system

https://doi.org/10.1016/j.toxrep.2018.04.003

Kathy Fowlera, Wanda Fieldsa, Victoria Hargreavesb, Lesley Reeveb, Betsy Bombicka,
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA2
b Covance Laboratories Ltd., North Yorkshire, UK

The article shows the successfull qualification of the Vitrocell VC10 smoking robot. The robot was used to  expose bacterial cells to cigarette whole smoke generated from 3R4F reference cigarettes and Eclipse cigarettes. A comparison was made between whole smoke from a tobacco heating and a combustible cigarette.

 

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Comments (0) Number of views (375)

5. Apr. 2018

A Case Study for the Comparison of In Vitro Data Across Multiple Aerosol Exposure Studies with Extrapolation to Human Dose

DOI: 10.1089/aivt.2017.0042

David Thorne, Emma Bishop, Linsey Haswell, and Marianna Gaca
British American Tobacco Group R&D, Southampton, United Kingdom

This case study has demonstrated that dosimetry techniques using particulate markers can allow in vitro biological data generated on five independent aerosol exposure system studies, under contrasting experimental designs and with different cell types, to be directly compared.

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28. Mar. 2018

Characterization of a Vitrocell VC1 Using Nicotine Dosimetry: An Essential Component Toward Standardized In Vitro Aerosol Exposure of Tobacco and Next Generation Nicotine Delivery Products

DOI: 10.1089/aivt.2018.0001
Holger Behrsing,1 Mario Aragon,1 Jason Adamson,2 Devin Sheehan,1 Marianna Gaca,2 Rodger Curren,1 and Erin Hill1
1Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
2R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

 

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15. Mar. 2018

Biological effects of cigarette smoke and novel tobacco product vapor on human organotypic bronchial epithelial cultures analyzed using an in vitro aerosol exposure system

SOT 2018, San Antonio, USA

Shinkichi Ishikawa, Kazushi Matsumura, Nobumasa Kitamura, Kanae Ishimori, Yuichiro Takanami, Shigeaki Ito
Scientific Product Assessment Center, Japan Tobacco Inc., 6-2, Umegaoka, Aoba-Ku, Yokohama, Japan

This Poster shows the exposure of nicotine and TPM in the mainstream aerosols from 3R4F and novel tobacco vapor on MucilAir™ cells using the Vitrocell® exposure system. The results are shown in cytotoxicity, IL-8, IL-1ß and gene expression.

 

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11. Mar. 2018

A setup to test toxicity of alcohol inhalation from consumer products

User Group Meeting 2018

Dr. Monique Manche1, Dr. Fabrice Nesslany2, Tobias Krebs3
1Laboratoires Anios, 59260 Lille-Hellemmes, France
2Laboratoire de toxicologie génétique, Institut Pasteur de Lille, 59000 Lille, France
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

Genotoxicity tests (in vitro micronucleus test and comet assay) have been conducted on p53 competent pulmonary cells, with alcohol administration as vapours to obtain data under conditions more representative of actual exposure conditions via the pulmonary route.

 

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11. Mar. 2018

Update on FTIR integration

User Group Meeting 2018

Dr. Martin Kieninger1, Tobias Krebs2

1ANSYCO GmbH, Gasmet Group, 76131 Karlsruhe, Germany

2VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

The poster shows a reliable, easy to use online analysis tool with fast response and a wide range of components due to Calcmet library. Ideal for quality control and dose monitoring.


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Comments (0) Number of views (286)

11. Mar. 2018

Photometer Calibration Method

User Group Meeting 2018

Johannes Sood

VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The poster shows a reliable method for the photometer calibration.

 

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Comments (0) Number of views (723)

11. Mar. 2018

VITROCELL® Cloud MAX

User Group Meeting 2018

Patrick Weindl1, Dr. Otmar Schmid2,

1VITROCELL Systems GmbH, 79183 Waldkirch, Germany
2Helmholtz Center Munich, Germany

The poster shows the design for smallest nebulization volumes and high deposition efficiency.

 

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Comments (0) Number of views (325)

11. Mar. 2018

Update: Deposition Enhancement via Thermophoresis

User Group Meeting 2018

Dr. Dirk Brossell

Federal Institute for Occupational Safety and Health (BAuA), Unit 4.5, 10317 Berlin, Germany

The poster demonstrates the particle deposition via thermal precipitation (particle precipitation by thermophoresis) on cells hosted at the air-liquid interface (ALI).
Advantages of thermophoresis
• Diffusion-like mechanism, gentle particle deposition 
• Independent from particle size (for nanoparticles)
• Homogenous deposition density profile 
• No need to manipulate aerosol properties for deposition (charge, size)

 

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Comments (0) Number of views (244)

11. Mar. 2018

Characterization of a Vitrocell VC1 Using Nicotine Dosimetry: An Essential Component Towards Standardised In Vitro Aerosol Exposure of Tobacco and Next Generation Nicotine Delivery Products

IIVS at SOT 2018

D. Sheehan1, M. Aragon1, J. Adamson2, M. Gaca2, H. Raabe1, and H. Behrsing1.
1Institute for In Vitro Sciences, Inc., Gaithersburg, MD; and 2British American Tobacco, Southampton, United Kingdom.

This study demonstrates successful Vitrocell VC1 aerosol generation and delivery across multiple nicotine product categories (3R4F cigarette smoke, e-liquid and THP), as characterised using nicotine as a dosimetry marker. The data suggests the VC1 can reproducibly generate and deliver tobacco product and NGP aerosols for future in vitro assessment and matches the performance of reported exposure systems.

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Comments (0) Number of views (545)
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