Advanced in vitro exposure systems.

17. Oct. 2020

Cell‑specific toxicity of short‑term JUUL aerosol exposure to human bronchial epithelial cells and murine macrophages exposed at the air–liquid interface

https ://doi.org/10.1186/s1293 1-020-01539-1


Rakeysha Pinkston1,2, Hasan Zaman2, Ekhtear Hossain2, Arthur L. Penn2 and Alexandra Noël2
1 Department of Environmental Toxicology, College of Sciences and Engineering, Southern University and A&M College, Baton Rouge, LA 70813, USA. 
2 Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA.

 

There are thousands of flavors and flavoring combinations of e-liquids on the market with the potential to produce harmful effects when aerosolized through an ENDS device. While more research is needed regarding the potential toxicity associated with inhaling flavoring additives in combination with nicotine salt for future regulation of ENDS products, the present study provides laboratory-based evidence that should be considered regarding regulation of nicotine salt-based products.

 

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8. Oct. 2020

Alternative air–liquid interface method for inhalation toxicity testing of a petroleum-derived substance

https://doi.org/10.1016/j.mex.2020.101088


Verstraelen Sandra a , Jacobs An a , Van Laer Jo a , Van Deun Masha b , Bertels Diane b , Hilda Witters a , Remy Sylvie a , c , Geerts Lieve a , Deferme Lize d , Frijns Evelien a
a VITO NV (Flemish Institute for Technological Research), Unit HEALTH, Mol, Belgium 
b VITO NV, Unit SCT (Separation and Conversion Technology), Mol, Belgium 
c Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium 
d ExxonMobil Petroleum and Chemical B.V., Machelen, Belgium

 

A549 cells were exposed to gasoline at the ALI by passive dosing, an approach that is already used in aquatic toxicity testing. This showes a clear dose-dependent biological response. This in vitro -based new approach methodology might be promising for inhalation toxicity testing of (semi-)volatile complex substances.

 

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29. Sep. 2020

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

https://doi.org/10.1007/s11626-020-00517-7


Xuefei Cao1, Jayme P. Coyle2, Rui Xiong1, Yiying Wang1, Robert H. Heflich1, Baiping Ren1, William M. Gwinn3, Patrick Hayden4, Liying Rojanasakul2

1 Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Rd., AR Jefferson, USA
2 Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers forDisease Control and Prevention,Morgantown,WV, USA
3 Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Durham, NC, USA
4 BioSurfaces Inc., Ashland, MA, USA


One important element for validating any new assay for making regulatory decisions is determining its performance relative to an accepted standard. Conducting in vivo inhalation toxicity studies using whole-body or nose-only exposure systems is expensive and time-consuming and typically requires a large number of animals. The goal of using alternative methods, like human in vitro ALI airway cultures, ultimately is to replace inhalation toxicity testing in animals with in vitro approaches. Transition from animal- to human-based models is ultimately expected to lead to faster and better predictive toxicity assessments and therapeutic development at lower cost.  This study shows the development and validation of alternative in vitro methods for acute toxicity testing, including acute inhalation toxicity testing.
 

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28. Sep. 2020

NanoCELL - Comprehensive characterization and human toxicological assessment of cellulose nanocrystals along their life cycle for reliable risk assessment

Yvonne Kohl1, Roland Drexel2, Christine Herrmann3, Stephan Dähnhardt-Pfeiffer4, Siegfried Fürtauer5, Michelle Hesler1, Christoph Metzger3, Marielle Fink6, Dominik Selzer7, Thorsten Lehr7, Tobias Krebs6, Sven van Lengen8, Sylvia Wagner1, Hagen von Briesen1, Felix Grimm9, Petra Weißhaupt10, Heiko Briesen3, Florian Meier2


1Fraunhofer-Institut für Biomedizinische Technik IBMT, Sulzbach, Germany; 
2Postnova Analytics GmbH, Landsberg, Germany, 
3Technische Universität München, Lehrstuhl für Systemverfahrenstechnik, Freising, Germany 
4Microscopy Services Dähnhardt GmbH, Flintbek, Germany
5Fraunhofer-Institut für Verfahrenstechnik und Verpackung IVV, Freising, Germany
6VITROCELL Systems GmbH, Waldkirch, Germany
7Universität des Saarlands, Klinische Pharmazie, Saarbrücken, Germany 
8GRÜNPERGA Papier GmbH, Grünhainichen, Germany 
9INFIANA Germany GmbH & Co. KG, Forchheim, Germany
10Umweltbundesamt, Dessau-Roßlau, Germany

 

This poster shows the hazard studies on the effect of oral and pulmonary CNC uptake. Therefore a miniaturized cloud exposure system has been developed to create an in vitro model simulating the lung and the GI tract.

 

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16. Sep. 2020

Quartz crystal microbalances (QCM) are suitable for real-time dosimetry in nanotoxicological studies using VITROCELL®Cloud cell exposure systems

https://doi.org/10.1186/s12989-020-00376-w


Yaobo Ding1,2 , Patrick Weindl1,2,3, Anke-Gabriele Lenz1,2, Paula Mayer1,2, Tobias Krebs3 and Otmar Schmid1,2
1Institute of Lung Biology and Disease, Helmholtz Zentrum München, 85764 Neuherberg, Germany
2Comprehensive Pneumology Center, Munich (CPC-M) - Member of the German Center for Lung Research (DZL), 81377 Munich, Germany
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany.

 

This study provides evidence that QCMs are suitable for real-time dosimetry in particle toxicology studies with cell cultures under air-liquid interface conditions. An experimental method for determination of LoD (lower limit of detection), accuracy and precision of QCMs using a fluorescent tracer (fluorescein salt) was presented and applied to the QCMs integrated in the VITROCELL® Cloud 6 and Cloud 12 aerosol-cell exposure systems.

 

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27. Aug. 2020

Comparative anti-inflammatory effect of curcumin at air-liquid interface and submerged conditions using lipopolysaccharide stimulated human lung epithelial A549 cells

https://doi.org/10.1016/j.pupt.2020.101939


Yue Hu a,b,c, Yunhua Sheng c,e, Xiaoli Ji c,e, Ping Liu c,e, Liming Tang c,e, Gang Chen a,Guiliang Chen b,d,
a School of Pharmacy, Fudan University, Shanghai, China
b China State Institute of Pharmaceutical Industry, Shanghai, China
c Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, China
d Shanghai Center for Drug Evaluation and Inspection, Shanghai, China
e NMPA Key Laboratory for Quality Analysis of Chemical Drug Preparations, Shanghai, China

 

In this study, the ALICE-CLOUD system was used as an easy-to-use and dosimetrically accurate ALI system for efficient delivery of the curcumin aerosol to the cells. It has been used to assess not only the respiratory effect of nanoparticles and chemicals after single or repeated exposure, but also the biokinetics of aerosolized drugs. Conventional ALI systems were mainly developed for toxicity study of inhaled substances. Compared with other systems, the ALICE-CLOUD system is not only technically simpler, easier to handle, but also has higher drug delivery efficiency and shorter exposure time. The short exposure time of the ALICE-CLOUD system was similar to the actual administration pattern of the inhaled drugs, which is suitable for preclinical development of aerosolized drugs and cellular response mechanisms for inhalation therapy.

 

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24. Aug. 2020

Product News 08/2020

VITROCELL® Cloud Alpha 96

96-well Air/Liquid Interface (ALI) exposure. For reliable high throughput testing of cell cultures.

The VITROCELL® Cloud Alpha 96 is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines high throughput testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12- and 24-well). It’s functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the air/liquid interface have never been easier.

VITROCELL® Cloud Alpha 96

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15. Aug. 2020

Development of an in vitro approach to point-of-contact inhalation toxicity testing of volatile compounds, using organotypic culture and air-liquid interface exposure

https://doi.org/10.1016/j.tiv.2020.104968

Artik Mistry, Larry E. Bowen, Michael W. Dzierlenga, Jessica K. Hartman, Scott D. Slattery
ScitoVation, LLC, Durham, NC 27713, United States
 

In this project, an incremental approach to assay development, taking small steps that built understanding of the biology and confidence in the methods were taken to attempted. Focused on general cell health assays (viability,cytotoxicity, TEER), as well as a mechanism-of-action-specific assay (GSH depletion), we found all assays to be sufficiently sensitive over the full range of possible values for both cell culture models (BEAS-2B cell and EpiAirway cultures)
 

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27. Jul. 2020

Vitrocell® holder systems for e-cigarettes

Secure and tight connection of any device to the smoking machine.

New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

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27. Jul. 2020

An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols

doi:10.3390/ijms21155335


Hana Barosova 1,2 , Bedia Begum Karakocak 1 , Dedy Septiadi 1 , Alke Petri-Fink 1,3, Vicki Stone 4 and Barbara Rothen-Rutishauser 1,
1 BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, 
2 Institute of Experimental Medicine of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
3 Department of Chemistry, University of Fribourg, 1700 Fribourg, Switzerland
4 Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK


The in vitro co-culture model consisting of three human cell lines were exposed at the ALI using the VITROCELL® Cloud system, equiped with QCM allowing to measure and record the deposited dose online. The nebulizer was ideal for the aerosols to suffciently mix within the entire chamber, hence resulting in uniform droplet deposition. This study shows, that this model is not limited to testing potentially hazardous nanomaterials to human cell line co-culture models.

 

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