Advanced in vitro exposure systems.

2. Feb. 2019

Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model.

DOI: 10.1016/j.yrtph.2019.01.036

Czekala L1, Simms L2, Stevenson M2, Tschierske N2, Maione AG3, Walele T2.
1 Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK. Electronic address: Lukasz.Czekala@uk.imptob.com.
2 Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK.
3 MatTek, 200 Homer Ave., Ashland, MA, USA.

In this research article a respiratory epithelial model, EpiAirway, was used to examine the biological effects of nicotine-containing blu PLUS + e-cigarettes, in comparison to conventional cigarette smoke. Tissues were exposed at the air-liquid interface to cigarette smoke or e-cigarette aerosol generated using a VITROCELL VC1 smoking/vaping robot. 

 

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20. Dec. 2018

Establishing the Vitrocell® Powder Chamber as a particle size-selective platform for in vitro dry powder testing

S. Steiner1 , M. Hittinger2 , K. Knoth2 , H. Gross2 , S. Frentzel1 , A. Kuczaj1 , J. Hoeng1 , T. Krebs3 , M. Peitsch1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland
2 PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany
3 Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

The poster shows the use of the Vitrocell® Powder Chamber for dry powder testing by online monitoring of the powder mass deposition, by QCMs Nicotine quantification, particle number deposition and particle size distribution.

 

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18. Dec. 2018

Development of a quantitative method for assessment of dose in in vitro evaluations using a VITROCELL® VC10® smoke exposure system

https://doi.org/10.1016/j.tiv.2018.12.010

Brian M. Keysera, Robert Leverettea, Kathy Fowlera, Wanda Fieldsa, Victoria Hargreavesb, Lesley Reeveb, Betsy Bombicka
a RAI Services Company1, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
b Covance Laboratories Ltd., North Yorkshire, UK

The aims of this study were to investigate dosimetry during whole smoke exposure in the VITROCELL® VC10® smoking robot using QCMs and to determine whether photometers could be used for concurrent assessment of dose during in vitro whole smoke exposures.

 

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28. Oct. 2018

Big tobacco embraces in vitro toxicology

Manufacturers invest in nonanimal tests to evaluate health risks of e-cigarettes and other next-generation tobacco products

c&en, Volume 96, Issue 43

Britt E. Erickson

The complexity of next-generation tobacco products has increased by a number of factors since FDA was given responsibility to regulate tobacco. Testing e-liquids with animals is not practical, economically and not that accurate. The Vitrocell system generates airborne materials from test products, such as smoke from reference cigarettes and vapor from e-cigarettes, in a manner that simulates how a person would puff. This artcile shows, how IIVS scientists use human donor tissues, obtained from organs, to create cell cultures and in vitro test systems.


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26. Jul. 2018

Hybrid Lipid/Polymer Nanoparticles for Pulmonary Delivery of siRNA: Development and Fate Upon In Vitro Deposition on the Human Epithelial Airway Barrier

DOI: 10.1089/jamp.2017.1364

Ivana d’Angelo, PhD,1 Gabriella Costabile, PhD,2,3 Estelle Durantie, PhD,3 Paola Brocca, PhD,4 Valeria Rondelli, PhD,4 Annapina Russo, PhD,5 Giulia Russo, PhD,5 Agnese Miro, PharmD,2 Fabiana Quaglia, PhD,2 Alke Petri-Fink, PhD,3 Barbara Rothen-Rutishauser, PhD,3 and Francesca Ungaro, PhD2
1 Di.S.T.A.Bi.F., University of Campania ‘‘Luigi Vanvitelli,’’ Caserta, Italy.
2 Laboratory of Drug Delivery, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.
3 Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
4 Applied Physics, Department of Medical Biotechnology and Translational Medicine, University of Milano, Milano, Italy.
5 Laboratory of Biochemistry, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.

The Vitrocell Cloud was used for the exposure to aerosolized nanoparticles on a in vitro three-dimensional cell cluture, mimicking the human epithelial airway barrier comprising human bronchial epithelial cells,human blood monocyte-derived macrophages and dendritic cells to show the optimized siRNA transport and delivery at lung.

 

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9. Jul. 2018

Delivery efficiencies of constituents of combustion-derived aerosols across the air-liquid interface during in vitro exposures.

DOI: 10.1016/j.tiv.2018.06.024

Steiner S, Diana P, Dossin E, Guy P, Vuillaume G, Kondylis A, Majeed S, Frentzel S, Hoeng J

PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland.

Highlights
•Different smoke constituents are delivered with different efficiencies during in vitro exposures.
•This indicates considerable differences in the composition of the applied and the absorbed smoke.
•The composition of the applied smoke is of limited value for describing the exposure.

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30. Jun. 2018

Characterization and modeling of aerosol deposition in Vitrocell® exposure systems - exposure well chamber deposition efficiency

DOI 10.1016/j.jaerosci.2018.06.015

 

Francesco Luccia, Nicolas D. Castrob, Ali A. Rostamib, Michael J. Oldhamb, Julia Hoenga, Yezdi B. Pithawallab, Arkadiusz K. Kuczajac

a Philip Morris International Research & Development, Philip Morris Products S.A. (part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland
b Altria Client Services LLC, Center for Research and Technology, Richmond, VA 23219, USA
c Multiscale Modeling & Simulation, Dept. of Applied Mathematics, University of Twente, P.O.Box 217, 7500 AE Enschede, The Netherlands

This paper successfully presents the geometry and characteristic dimensions of the two exposure systems: Vitrocell 24/48 and Vitrocell AMES 48. A computational modeling of aerosol transport and deposition shows the deposition efficiency.


 

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6. Jun. 2018

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

DOI: 10.2217/nnm-2017-0358

Savvina Chortarea1,2, Kleanthis Fytianos1,3,4, Laura Rodriguez-Lorenzo1, Alke Petri-Fink1,5 & Barbara Rothen-Rutishauser1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
2Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
3Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
4Department of Clinical Research, University of Bern, Bern, Switzerland
5Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

 

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1. Jun. 2018

Charakterisierung von Partikelemissionen aus dem Trennschleifprozess von kohlefaserverstärktem Beton (Carbonbeton)

Autoren:
Hillemann, L.; Stintz, M.; Streibel, T.; Zimmermann, R.; Öder, S.; Kasurinen, S.; di Bucchianico, S.; Kanashova, T.; Dittmar, G.; Konzack, D.; Große, S.; Rudolph, A.; Berger, M.; Krebs, T.; Saraji-Bozorgzad, M. R.; Walte, A.

Die Wirkung von Emissionen aus dem Trennen von Carbonbeton werden in einer Kombination aus Trennstaubgenerator, Verdünnungssystem und Expositionssystem an Zellkulturen (A549 und THP-1) gegenüber den entstehenden Stäuben bestimmt (Zytotoxizität).

 

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26. May. 2018

Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients

DOI: 10.1080/17435390.2018.1472310

Beyeler S1,2, Chortarea S3,4, Rothen-Rutishauser B3, Petri-Fink A3, Wick P4, Tschanz SA5, von Garnier C1,2, Blank F1,2.

1 Department of BioMedical Research , University of Bern , Bern , Switzerland.
2 Department of Pulmonary Medicine , University Hospital of Bern , Bern , Switzerland.
3 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg , Switzerland.
4 Laboratory for Particles-Biology Interactions, Empa Swiss Federal Laboratories for Material Science and Technology , St. Gallen , Switzerland.
5 Institute of Anatomy, University of Bern , Bern, Switzerland.

This paper shows the investigation of acute effects of occupational multi-walled carbon nanotubes exposure in primary epithelial lung cell cultures from potentially susceptible individuals with COPD.

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