Advanced in vitro exposure systems.

7. Oct. 2007

Toxicological investigation of nanoparticles - effects on human cells

EUROTOX. Amsterdam, Netherlands.

October 7-10, 2007

Authors
Letizia Farmer, Alexander Graff, Sandra Szameit, Eva Valic, Helga Tuschl

Austrian Research Centers GmbH - ARC, Life Sciences, Toxicology, A-2444 Seibersdorf, Austria

 

Differentiated macrophages were seeded on human lung epithelial cells for co-culturing. As endpoints for particle exposure cell viability (WST-1 assay), oxidative stress (DHRAssay) and pro-inflammatory cytokines (BDTM CBA-Assay) were evaluated.

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23. Jul. 2007

A modified Ames assay reveals the mutagenicity of native cigarette mainstream smoke and its gas vapour phase

ELSEVIER

Experimental and Toxicologic Pathology 58 (2007) 383-392

Authors
Michaela Aufderheide, Helga Gressmann

Department of In-Vitro Toxicology, Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany

 

Establishment of a new experimental approach for direct exposure of bacteria with actual generated smoke aerosol. This allowed us to investigate the mutagenic activity of native mainstream smoke on Salmonella Typhimurium strains. The new procedure of exposing bacteria directly to gases and complex mixtures offers new possibilities in the field of inhalation genotoxicology for the evaluation of genotoxicity in the Ames assay.

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23. Mar. 2007

Responses of differentiated primary human lung epithelial cells to exposure to diesel exhaust at an air-liquid interface

Experimental Lung Research

33:1, 27 - 51, 2007

Authors
JeanClare Seagrave, Sandy Dunaway, Jacob D. McDonald, and Joe L. Mauderly
Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA

Patrick Hayden
MatTek Corp, Ashland, Massachusetts, USA

Christine Stidley
Department of Internal Medicine, University of New Mexico, Albuquerque, New Mexico, USA

 

The study evaluated responses of human primary airway epithelial cells to diesel exhaust. This model may be useful for mechanistic and comparative toxicology studies.

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23. Mar. 2007

Cellular Mechanisms of Mainstream Cigarette Smoke-Induced Lung Epithelial Tight Junction Permeability Changes In Vitro

Inhalation Toxicology, 19:13–22, 2007

Authors
Dorian S. Olivera
Program in Toxicology, College of Pharmacy, University of New Mexico Health Sciences Center,
Albuquerque, New Mexico, and Lovelace Respiratory Research, Albuquerque, New Mexico, USA

Susan E. Boggs, Chris Beenhouwer, James Aden, and Cindy Knall
Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA

 

The goal of these studies was to identify the signal transduction pathways that regulate smoke-induced permeability. Using a physiologically relevant air-liquid interface exposure system, electrically tight monolayers of Calu-3 cell line were exposed to fresh, whole mainstream cigarette smoke. This exposure results in a regulated, dose-dependent loss of epithelial barrier function in the lung epithelial monolayers.

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31. Dec. 2006

Set-up of a dynamic in vitro exposure device for the study of indoor air pollutant effects on human derived cells

WIT Transactions on Biomedicine and Health

Vol 10, 2006

Authors
F. Pariselli, M. G. Sacco & D. Rembges

European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, Ispra, Italy


The aim of this work was to develop an in vitro exposure set-up for the assessment of the toxicity of single volatile chemicals and their mixtures on representative VOCs target tissues: skin (HaCaT) and lung (A549).

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31. Dec. 2006

Dynamic in-vitro Exposure of Human Derived Cells to Indoor Priority Pollutants

EUROPEAN COMMISSION, DIRECTORATE-GENERAL, Joint Research Centre

Report EUR 22285 EN, 2006

Authors
F. Pariselli, M. G. Sacco, D. Rembges

Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, Ispra, Italy

 

The aim of this work was to investigate toxicity of single volatile chemicals and their mixtures. Developing a reproducible in vitro dynamic exposure model where A549 and HaCaT cell lines representative of VOC’s target tissues (lungs and skin) were exposed in an airlifted interface to air pollutants. Several endpoints were evaluated: cytotoxicity (LDH), proliferation (WST-1), CFU (Colony Forming Units), inflammatory response (IL-8) and oxidative stress (ROS).

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31. Oct. 2005

Exposure System for Bioassays Measurement of the Toxicity of Airborne Nanoparticles

Research Center Karlsruhe

Authors
Dr. Paur, S. Mülhopt and Dr. Diabate

 

The exposure of human lung cells towards nano particles the correlation between particle diameter, material properties, number concentration and biological response could be determined.

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25. Apr. 2005

Experimental Assessment of the Toxicological Effects of Inhaled Complex Mixtures on the Respiratory System - Feasibility and Limitations

URBAN & FISCHER

Exp Toxic Pathol 2005, Supplement issued July, 2005

Workshop 23 - 25 April, 2005 in Barcelona, Spain


In this Workshop data were shown that demonstrate the response of cultured non-tumorigenic human bronchial epithelial cells (BEAS-2B) exposed to air pollution particles that were similar to the response seen in humans following exposure to the same particles. A presentation of the work with the air-liquid interface were given to compare in-vitro and in-vivo methods.

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31. Dec. 2004

Comparative Assessment of Toxicities of Mainstream Smoke from Commercial Cigarettes

Taylor & Francis

Inhalation Toxicology 16:1-10, 2004

Authors
Detlef Ritter, Jan W. Knebel, and Michaela Aufderheide

 

Online monitoring of generated smoke aerosols with respect to particulate and gas-phase components, and direct exposure of A549 cell line using exposure conditions relevant to human smoking.Results are evaluated in measuring glutathione and ED50.

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31. Dec. 2004

A modified CULTEX® system for the direct exposure of bacteria to inhalable substances

URBAN & FISCHER

Exp Toxic Pathol 2004; 55: 451-454

Authors
Michaela Aufderheide and Ulrich Mohr

 

Establishment of a novel experimental approach using an exposure device based on the cell exposure system CULTEX. This allows to investigate chemically and physically unchanged atmospheres like mainstream cigarette smoke by exposing bacteria of Salmonella typhimurium strains directly on the surface of culture media.

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Comments (0) Number of views (1333)
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