Advanced in vitro exposure systems.

10. Mar. 2008

The Response of a Co-culture Lung Model to Fine and Ultrafine Particles of Incinerator Fly Ash at the Air-liquid Interface

ATLA

ATLA, Issue 36, pp. 285-298, 2008

Authors
Silvia Diabaté, Sonja Mülhopt, Hanns-Rudolf Paur and Harald F. Krug

Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein - Leopoldshafen, Germany

 

BEAS-2B cells were exposed to an aerosol, generated from fly ash. The cells were co-cultured with differentiated THP-1 macrophages growing on Transwell inserts. Analyses of cell viability and IL-8 release were performed.

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Comments (0) Number of views (1002)

10. Mar. 2008

Exposure of a multi-cellular in vitro model of human airway tissue to the whole cigarette smoke

Respiratory and Critical Care Medicine

Vol 177 (Abstracts Issue): pA728 (2008)

Authors
Walsh, P.T., Chalupowicz, D.G., Frankowski, R., Barnette, M.

Discovery Biology, Respiratory CEDD, GlaxoSmithKline, Upper Merion, PA19406, USA

 

HBE cells were exposed in a single and repeated whole smoke situation. The cells were analysed for changes in gene expression (RT-PCR) and cell viability was confirmed using the MTT assay and release of IL-1, IL-6 and IL-8.

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10. Mar. 2008

Cellular responses of primary human bronchial epithelial cells to whole cigarette smoke exposure

Respiratory and Critical Care Medicine

Vol 177 (Abstracts Issue): pA199 (2008)

Authors
Chalupowicz, D.G., Frankowski, R., Sanchez, Y., Kou, J., Barnette, M., and Walsh, P.T.

GlaxoSmithKline


Primary human bronchial epithelial cells were exposed in a single and repeated whole smoke situation. The cells were analysed for changes in gene expression (RT-PCR) and cell viability was confirmed using the MTT assay and release of IL-1, IL-6 and IL-8.

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31. Oct. 2007

In Vitro Testing of Inhalable Fly Ash at the Air Liquid Interface

Advanced Environmental Monitoring

October 2007, pp 402-414

Authors
Sonja Mülhopt, Hanns-Rudolf Paur, Silvia Diabaté and Harald F. Krug

Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein - Leopoldshafen, Germany

 

Exposure experiments with the fine and ultrafine fraction of fly ash on human lung cells in the air-liquid interface have shown an increased release of IL-8. 

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Comments (0) Number of views (1095)

7. Oct. 2007

Toxicological investigation of nanoparticles - effects on human cells

EUROTOX. Amsterdam, Netherlands.

October 7-10, 2007

Authors
Letizia Farmer, Alexander Graff, Sandra Szameit, Eva Valic, Helga Tuschl

Austrian Research Centers GmbH - ARC, Life Sciences, Toxicology, A-2444 Seibersdorf, Austria

 

Differentiated macrophages were seeded on human lung epithelial cells for co-culturing. As endpoints for particle exposure cell viability (WST-1 assay), oxidative stress (DHRAssay) and pro-inflammatory cytokines (BDTM CBA-Assay) were evaluated.

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23. Jul. 2007

A modified Ames assay reveals the mutagenicity of native cigarette mainstream smoke and its gas vapour phase

ELSEVIER

Experimental and Toxicologic Pathology 58 (2007) 383-392

Authors
Michaela Aufderheide, Helga Gressmann

Department of In-Vitro Toxicology, Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany

 

Establishment of a new experimental approach for direct exposure of bacteria with actual generated smoke aerosol. This allowed us to investigate the mutagenic activity of native mainstream smoke on Salmonella Typhimurium strains. The new procedure of exposing bacteria directly to gases and complex mixtures offers new possibilities in the field of inhalation genotoxicology for the evaluation of genotoxicity in the Ames assay.

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Comments (0) Number of views (1165)

23. Mar. 2007

Responses of differentiated primary human lung epithelial cells to exposure to diesel exhaust at an air-liquid interface

Experimental Lung Research

33:1, 27 - 51, 2007

Authors
JeanClare Seagrave, Sandy Dunaway, Jacob D. McDonald, and Joe L. Mauderly
Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA

Patrick Hayden
MatTek Corp, Ashland, Massachusetts, USA

Christine Stidley
Department of Internal Medicine, University of New Mexico, Albuquerque, New Mexico, USA

 

The study evaluated responses of human primary airway epithelial cells to diesel exhaust. This model may be useful for mechanistic and comparative toxicology studies.

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23. Mar. 2007

Cellular Mechanisms of Mainstream Cigarette Smoke-Induced Lung Epithelial Tight Junction Permeability Changes In Vitro

Inhalation Toxicology, 19:13–22, 2007

Authors
Dorian S. Olivera
Program in Toxicology, College of Pharmacy, University of New Mexico Health Sciences Center,
Albuquerque, New Mexico, and Lovelace Respiratory Research, Albuquerque, New Mexico, USA

Susan E. Boggs, Chris Beenhouwer, James Aden, and Cindy Knall
Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA

 

The goal of these studies was to identify the signal transduction pathways that regulate smoke-induced permeability. Using a physiologically relevant air-liquid interface exposure system, electrically tight monolayers of Calu-3 cell line were exposed to fresh, whole mainstream cigarette smoke. This exposure results in a regulated, dose-dependent loss of epithelial barrier function in the lung epithelial monolayers.

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Comments (0) Number of views (1095)

31. Dec. 2006

Set-up of a dynamic in vitro exposure device for the study of indoor air pollutant effects on human derived cells

WIT Transactions on Biomedicine and Health

Vol 10, 2006

Authors
F. Pariselli, M. G. Sacco & D. Rembges

European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, Ispra, Italy


The aim of this work was to develop an in vitro exposure set-up for the assessment of the toxicity of single volatile chemicals and their mixtures on representative VOCs target tissues: skin (HaCaT) and lung (A549).

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Comments (0) Number of views (1058)

31. Dec. 2006

Dynamic in-vitro Exposure of Human Derived Cells to Indoor Priority Pollutants

EUROPEAN COMMISSION, DIRECTORATE-GENERAL, Joint Research Centre

Report EUR 22285 EN, 2006

Authors
F. Pariselli, M. G. Sacco, D. Rembges

Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, Ispra, Italy

 

The aim of this work was to investigate toxicity of single volatile chemicals and their mixtures. Developing a reproducible in vitro dynamic exposure model where A549 and HaCaT cell lines representative of VOC’s target tissues (lungs and skin) were exposed in an airlifted interface to air pollutants. Several endpoints were evaluated: cytotoxicity (LDH), proliferation (WST-1), CFU (Colony Forming Units), inflammatory response (IL-8) and oxidative stress (ROS).

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Comments (0) Number of views (1090)
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