Advanced in vitro exposure systems.

Air/Liquid Culture and Exposure Technique: Preliminary Results of a Prevalidation Study

3. Sep. 2009

World Congress on Alternatives and Animal Use in the Life Sciences. Rome, Italy.

August 30 - September 3, 2009

Smirnova, L.1, Liebsch, M.1, Tharmann, J.1, Pirow, R.1, Luch, A.1, Bauer, M.2, Graebsch, C.2, Linsel, G.3, Siemers, R.3, Berger-Preiß, E.4, Kock, H.4, Oertel, A.4, Riiter, D.4, Knebel, J.4

1 Federal Institute for Risk Assessment (BfR), Unit 37, Centre for Alternative Methods to Animal Experiments, ZEBET, Berlin, Germany
2 Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany
3 Federal Institute for Occupational Safety and Health (BAuA), Berlin, Germany
4 Fraunhofer Institute of Toxicology and Experimental Medicine (ITEM), Hannover, Germany


The aim of this study is to determine the in vitro vs. in vivo dose-response relationships. A549 cell were grwon in the air-liquid interface and exposed to NO2, SO2, formaldehyde and ozone. The results are measured in cytotoxicity (viability) and genotoxicity (COMET assay). The project has proved satisfying. transferability of the test method.

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