Advanced in vitro exposure systems.

For sequential and multiple exposures to liquid aerosols


This system is specifically designed for dose-controlled and spatially uniform deposition of liquid aerosols on cells cultured at the air/liquid interface. The aerosol is applied for a short time of approx. 3 – 4 minutes.

VITROCELL® Cloud System Phase 1
Phase 1 - Emission of Cloud
VITROCELL® Cloud System Phase 2
Phase 2 - Homogeneous Mixing
VITROCELL® Cloud System Phase 3
Phase 3 - Gravitational Settling

The VITROCELL® Cloud can be used for aerosols generated from liquids and suspensions. Possible fields of application are screening for inhaled drugs and toxicity testing of inhaled substances including nanoparticle suspensions. There is a choice among 4 options of Aeroneb® ­nebulizers:

  • 4.0 – 6.0 µm
  • 2.5 – 6.0 µm
  • 2.5 – 4.0 µm
  • 10 µm (special version)

The Cloud aerosol chamber is made of Polycarbonate. The VITROCELL® Cloud SEQ represents another evolution of the ­well-known Cloud system. Keeping functional process parameters according to established devices like the VITROCELL® Cloud 6, 12 or 24 it enhances these devices even further. It offers the possibility to sequentially expose rows of inserts to create dose-response effects via multiple exposures in one experiment. The concentration of nebulized liquid is kept at the same level for each nebulisation and the cell cultures under comparable conditions.

VITROCELL® Cloud SEQ 24 without insert cover

VITROCELL® Cloud SEQ 24 without insert cover

first exposure
VITROCELL® Cloud SEQ 24 with two insert covers

VITROCELL® Cloud SEQ 24 with two insert covers

second exposure
VITROCELL® Cloud SEQ 24 with four insert covers

VITROCELL® Cloud SEQ 24 with four insert covers

third exposure


  • Up to 6 doses @ 4 replicates for 24-well sized inserts
  • New exposure system for liquid aerosols
  • High droplet output rate – cloud dynamics
  • No external air-flow required (simple)
  • No humidity control required
  • Dose-controlled and spatially uniform aerosol deposition
  • Small residual volume in nebulizer reservoir
  • Low insert-to-insert variabilities
  • Easy handling
  • Clinically relevant
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