Advanced in vitro exposure systems.

VITROCELL® Cloud Alpha MAX

Smallest nebulization volumes and high deposition efficiency

VITROCELL® Cloud Alpha MAX

The VITROCELL® Cloud Alpha MAX is our newest innovation in the Cloud family and ­presents a great leap forward in ­automated exposure of cell cultures. It combines high ­throughput testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12- and 24-well). It’s functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the Air/Liquid Interface have never been easier.

The new Cloud Alpha MAX  series was developed as a result of numerous customer requests and is now capable to expose mammalian cell cultures in 6-, 12- or 24-well sized cell culture inserts. All commercial brands are supported.

The Cloud system is suitable for nebulization of solutions and ­suspensions. Possible fields of application are screening of inhaled drugs, toxicity testing of inhaled substances such as chemicals or nanoparticles and virus research.

 

Exposure at the Air/Liquid Interface

In response to the scientific need to expose in physiologically relevant conditions, the VITROCELL® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired inter­action of the formerly airborne substances with the culture media.

Air/Liquid Cultivation and Exposure in Exposure Module

Cell systems cultivated on membrane inserts are exposed at the Air/Liquid Interface (ALI) so that the test substances are received directly. This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.

 

Figure: Air/Liquid Cultivation and Exposure in Exposure Module:
A: Direct and controlled ­exposure of test ­atmosphere to cells
B: Cells on membrane
C: Media below cells

 

 

Ideal for small quantities of test substance

Air/Liquid Cultivation and Exposure in Exposure ModuleThe VITROCELL® Cloud Alpha MAX is designed for lower nebulization volumes and very high deposition efficiency. This is important when only small quantities of material are available or when expensive test substances need to be tested. The non-cell exposed area is reduced significantly by direct nebulization into exposure chambers with reduced chamber volume. A set of 3 nebulizers allows for simultaneous testing of 3 concentrations, or a combination of Microbalance, control liquid and suspension of the test substance.

 

How the Vitrocell ® Cloud works

After pipetting the suspension, exposure to the cell cultures takes place in three phases:

VITROCELL® Cloud System Step 1 - Phase 1

Phase 1: Emission of Cloud

VITROCELL® Cloud System Step 1 - Phase 2

Phase 2: Homogeneous Mixing

VITROCELL® Cloud System Step 1 - Phase 3

Phase 3: Gravitational Settling

 

Features

  • ≤ 40 µl per exposure and well
  • High deposition efficiency
  • Cloud dynamics by vibrating mesh nebulizers
  • Easy handling with no external air-flow required
  • High reproducibility
  • Air Liquid Interface (ALI) exposure
  • Direct and efficient guidance of substance exposure to the cells
  • 80, 40, 10 mm tube length
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