In vitro ASSAY GUIDE Examples Category Module type VITROCELL 6 CF VITROCELL 6 Cloud 6 / MAX VITROCELL 12 CF VITROCELL 12/6 VITROCELL 12/6 CF VITROCELL 12/12 Cloud 12 VITROCELL 24 Cloud 24 VITROCELL AMES VITROCELL SC Number of inserts 3, 4 or 6 6 or 48 3 or 4 6 6 12 24 or 48 3, 4 or 48 3 or 4 Insert type (size) 6-well 6-well 12-well 12-well 12-well 12-well 24-well 35 mm Petri Dish 35 mm Petri Dish Culture Media Supply Continuous Static Continuous Static Continuous Static Static -- -- Cytotoxicity Number of viable cells x x xx xx xx xx xx – – LDH release xx xx xx xx xx xx x – – NRU uptake x x xx xx xx xx xx – – MTT x x xx xx xx xx xx – – XTT x x xx xx xx xx xx – – MTS x x xx xx xx xx xx – – Proliferation WST-1 x x xx xx xx xx xx – – Protein level x x xx xx xx xx xx – – Cellular Stress ATP xx xx x x x x x – – Cellular ATP/ADP ratio xx xx x x x x x – – GSH xx xx x x x x x – – GSSG xx xx x x x x x – – GSSG/GSH xx xx x x x x x – – Oxidative Stress Lipid peroxidation (MDA test) xx xx x x x x x – – Inflammation Cytokines like IL8, IL6, IL12 x x xx xx xx xx x – – Genotoxicity Comet assay x x xx xx xx xx – – – AMES/number of revertants – – – – – – – xx – "omic“ technologies ARN Sample for microarray xx xx – – – – – – – ARN Sample for QRT PCR xx xx – – – – – – – Molecular Biology Protein Sample for Western Blot xx xx – – – – – – – Assays for Suspension Cells – – – – – – – – xx Legend: x = well suited xx = particularly well suited 11
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