Advanced in vitro exposure systems.

13. Jun. 2017

PETA science consortium donates $400K in VITROCELL in vitro test equipment

London -- Today, the PETA International Science Consortium Ltd. announced that it is providing equipment to laboratories working on human-relevant methods to replace the use of animals in inhalation testing. Valued at more than $400,000, VITROCELL Systems inhalation exposure machines were awarded to four institutions in the U.S., U.K., and Belgium that are leading the field of in vitro (non-animal) inhalation toxicology. These donations complement the Consortium's recent workshops and webinars focused on replacing inhalation testing in animals.

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15. Mar. 2017

New Products 2017 presented at VITROCELL® User Group Meeting

10 March 2017, Hilton Baltimore Inner Harbor Hotel, USA

Following the VITROCELL® User Group Meeting 2016 in New Orleans, international scientists from Canada, Europe, Japan, Korea and USA reconvened in March 2017.

The meeting offered an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange experiences in working with VITROCELL® devices and to meet fellow researchers. It was held prior to the International Society of Toxicology 56th Annual Meeting and ToxExpo – one of the largest international conferences related to toxicology.

Focus of the event was to exchange latest research results, to give an update on VITROCELL’s participation in major European and German research programs as well as the presentation of new products for 2017.

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22. May. 2017

Regulating temperature and relative humidity in air–liquid interface in vitro systems eliminates cytotoxicity resulting from control air exposures

DOI: 10.1039/c7tx00109f
Jose Zavala,a Rebecca Greenan,b Q. Todd Krantz,a David M. DeMarini,a Mark Higuchi,a M. Ian Gilmour a and Paul A. Whiteb

aNHEERL, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA.
bMechanistic Studies Division, Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario K1A 0K9, Canada.


The aim of this study was to test whether temperature and relative humidity has an influence on the cytotoxicity to BEAS-2B cells on a VITROCELL® 6 air-liquid interface system by measuring the viability (WST-1 cell proliferation assay) and lactate dehydrogenase (LDH) release.
Note from VITROCELL: We inform our customers about the possible need for humidification during installation and training. Among the need for humidification, possible other reasons for low clean air viability are clean air quality and cell handling procedures. Please see our website chapter on humidification for further information http://www.vitrocell.com/inhalation-toxicology/humidification

 

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15. May. 2017

Human Asthmatic Bronchial Cells Are More Susceptible to Subchronic Repeated Exposures of Aerosolized Carbon Nanotubes At Occupationally Relevant Doses Than Healthy Cells

DOI: 10.1021/acsnano.7b01992

Savvina Chortarea,1 Hana Barosova,1 Martin James David Clift,2 Peter Wick,3 Alke Petri-Fink,1,4 and Barbara Rothen-Rutishauser,1

1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, CH-1700 Fribourg, Switzerland
2In Vitro Toxicology Group, Swansea University Medical School, Swansea SA2 8PP, Wales, U.K.
3Laboratory for Materials-Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science and Technology, 9014 St Gallen, Switzerland
4Department of Chemistry, University of Fribourg, CH-1700 Fribourg, Switzerland

The aim of this study was to realistically mimic subchronic inhalation of multiwalled carbone nanotubes in vitro, using the air−liquid interface cell exposure system for aerosol exposures on reconstituted human bronchial tissue from healthy and asthmatic donors. Cytotoxicity, cell morphology, cilia beating frequency, mucociliary clearance, gene expression analysis of oxidative stress and (pro‑)inflammatory markers as well as (pro‑)inflammatory cytokine secretion were successfully observed.

 

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1. May. 2017

Proof of Concept of the VITROCELL® Dry Powder Chamber: A New In Vitro Test System for the Controlled Deposition of Aerosol Formulations

Marius Hittinger,1 Sarah Barthold,2 Lorenz Siebenbürger,1 Kilian Zäh,3 Alexander Gress,1 Sabrina Guenther,1 Birgit Wiegand,1 Carsten Boerger,1 Markus Berger,4 Tobias Krebs,4 Henrik Groß,1 and Claus-Michael Lehr1,5

1PharmBioTec GmbH, Saarbrücken, Germany
2Saarland University, Department of Pharmacy, Saarbrücken, Germany
3Scienceneering UG, Blieskastel, Germany
4Vitrocell Systems GmbH, Waldkirch, Germany
5Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), HZI, Saarbrücken, Germany

The VITROCELL® Dry Powder Chamber is designed for aerosol deposition of different sized particles separated by their time of flight. For the development of this new instrument salbutamol sulphate was successfully exposed to human pulmonary epithelial cell culture by sedimentation and diffusion in vitro.

 

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1. Mar. 2017

Characterization and Application of the VITROCELL VC1 Smoke Exposure System and 3D EpiAirway Models for Toxicological and e-Cigarette Evaluations

DOI: 10.1089/aivt.2016.0035
Wanda Fields,1 Anna Maione,2 Brian Keyser,1 and Betsy Bombick1

1RAI Services Company, Scientific and Regulatory Affairs, Winston-Salem, North Carolina.
2MatTek Corporation, Ashland, Massachusetts.

The aim of this study was to assess the effects of aerosol from two reference combustible cigarettes and two e-cigarettes by using the VC1 smoking machine with the air-liquid interface and to evaluate donor-to-donor variability of three EpiAirway tissue donors with the endpoints viability, barrier integrity, and gene promoter/expression regulation.


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31. Jan. 2017

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

DOI: 10.1089/aivt.2016.0025

Jason Adamson1,* Xiang Li2,* Huapeng Cui2, David Thorne1, Fuwei Xie2, and Marianna Gaca1
1British American Tobacco, R&D, Southampton, United Kingdom.
2Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, PR China.
*Joint first authors.

A VC 10 Smoking Robot was designated to look at the generated aerosol nicotine concentration from different e-cigarettes compared with the 3R4F reference cigarette in two different laboratories. The aerosol was trapped and the nicotine concentration was analysed by different kinds of mass spectometry.
Nicotine assessment across the tested e-cigarette categories showed consistent delivery of nicotine per puff within products and that the method was sensitive enough to detect different levels of nicotine across products. Good overlap in nicotine results were obtained in the two different laboratories.

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3. Jan. 2017

Development, qualification, validation and application of the neutral red uptake assay in Chinese Hamster Ovary (CHO) cells using a VITROCELL® VC10® smoke exposure system

Wanda Fields a, Kathy Fowler a, Victoria Hargreaves b, Lesley Reeve b, Betsy Bombick a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street,Winston-Salem, NC 27101, USA
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG1 3PY, UK

This study discribes the development, validation and application of the NRU assay in Chinese Hamster Ovary (CHO) cells, following exposure to fresh whole smoke generated with the VITROCELL®VC10®system. The VITROCELL® VC10® successfully provided consistent generation and delivery of whole smoke from a cigarette (3R4F) and a primarily “heat-not-burn” cigarette (Eclipse).

 

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30. Dec. 2016

The Society of Toxicology's 56th Annual Meeting and ToxExpo

March 13 - 15, 2017. Baltimore Convention Center - Baltimore, Maryland, USA

The Society of Toxicology 56th Annual Meeting and ToxExpo promises stimulating lectures and presentations on scientific breakthroughs, important education and professional training opportunities, and time to connect with old friends and create new collaborations.

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Categories: Exhibitions

Tags: congress

16. Dec. 2016

'Clean-burning' Fuels May be Worse for Your Lungs

Nala Rogers for 'Inside Science'

A report about artificial lung reveals surprising dangers from pellet burners and diesel-fueled ships at the University of Rostock and Helmholtz Zentrum München in Germany.

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