Advanced in vitro exposure systems.

In vitro exposure of organotypical 3D epithelial tissues to cigarette smoke as a potential alternative to rodent inhalation studies

10. Mar. 2011

SOT Washington DC, USA

March 06-10, 2011

Authors
Weisensee, D., Kurkowsky, B., Hebestreit, M., Wagner, S. and Schueller J. 

Philip Morris Research Laboratories GmbH, Cologne, Germany, Abstract #998

 

Here we describe an in vitro test system that examines the toxicological effects of single or repeated cigarette smoke exposure on human organo- typically reconstituted 3D epithelial tissues. These tissues were exposed at the air liquid interface to either fresh air or to mainstream smoke from the Reference Cigarette 3R4F in the VITROCELL® system. 

Abstract

Airway epithelium is the initial barrier affected by toxic gases or particles in the atmosphere, such as cigarette smoke (CS), which induces, among other things, inflammatory processes that can lead to COPD and tissue remodeling (Takizawa 2001, Yoshida 2007). Here we describe an in vitro test system that examines the toxicological effects of single or repeated CS exposure on human organo- typically reconstituted 3D epithelial tissues (EpiAirway™, MatTek).

Tissues were exposed at the air liquid interface to either fresh air or to mainstream smoke from the Reference Cigarette 3R4F in the VITROCELL® system (3-well or 24- well exposure chamber).

The objective of the study was to demonstrate whether exposure of human lung tissue to whole smoke in vitro can induce similar effects as observed in rodent inhalation studies with regard to selected gene and protein expression.

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