Advanced in vitro exposure systems.

Dynamic in-vitro Exposure of Human Derived Cells to Indoor Priority Pollutants

31. Dec. 2006

EUROPEAN COMMISSION, DIRECTORATE-GENERAL, Joint Research Centre

Report EUR 22285 EN, 2006

Authors
F. Pariselli, M. G. Sacco, D. Rembges

Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, Ispra, Italy

 

The aim of this work was to investigate toxicity of single volatile chemicals and their mixtures. Developing a reproducible in vitro dynamic exposure model where A549 and HaCaT cell lines representative of VOC’s target tissues (lungs and skin) were exposed in an airlifted interface to air pollutants. Several endpoints were evaluated: cytotoxicity (LDH), proliferation (WST-1), CFU (Colony Forming Units), inflammatory response (IL-8) and oxidative stress (ROS).

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