Advanced in vitro exposure systems.

Airway epithelium co-cultured with immune cells for a better assessment of the low dose effects of environmental pollutants

23. Sep. 2015

EUROTOX 13.-16. September 2015

Authors

Emilie RICQUEBOURG, Gabrielle VERNOUILLET, Isabelle MOMAS, Nathalie SETA, Sophie ACHARD

Public Health and Environment Laboratory, EA4064 - Paris Descartes University


Exposure of airborne pollutants to a 3D airway epithelial cell modell in the air-liquid interface to measure the epithelial thickness and the inflammatory respone with the IL-8 assay.

Winner of the price "AstraZeneca Young Investigator Prize for Innovation in Drug Discovery and Development"

PURPOSE
People are daily exposed to various pollutants present in airborne pollution. Many epidemiological studies revealed the existence of an association between indoor air pollution (aldehydes, VOCs, …) and the respiratory disorders. To study a such relationship, it is important to have toxicological elements to clarify the impact of these pollutants on cellular or tissue targets. Development of new in vitro models to assess the impact of environmental pollutants on human health is necessary. The present study investigated the pertinence to use a reconstituted epithelium, a 3D-model close to the human respiratory epithelium, co-cultured with immune cells, to assess the impact of environmental pollutants, such as volatile organic compounds (VOC), on biological activity.

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