Advanced in vitro exposure systems.

A novel approach to assess irritant or respiratory allergenic potential of chemicals in vitro

25. Aug. 2011

World Congress on Alternatives and Animal Use in the Life Sciences. Montréal, Canada.

August 21-25, 2011

Authors
Singal M.1, Vitale D.1, Smith L.1, Czollner K.2, Farmer L.2, Höpfner C.2, Satzer H-P.2, Schoeffmann S.2, Schwartz B.2, Weber E.2

1 Research Institute for Fragrance Materials, Inc., Woodcliff Lake, NJ, USA
2 Seibersdorf Labor GmbH, Seibersdorf, Austria

 

This article shows a method for the identification of chemical respiratory allergens. Two respiratory allergens were applied to a 3D co-culture cell system model at the air/liquid interface, mimicking the in-vivo situation in the lung. 
The results are shown in cytotoxicity (Propidium iodide, PI), Oxidative burst (Dihydrorhodamine assay, DHR), Pro- and antiinflammatory cytokines IL-2, IL-8, IL-10, IL-13, CD54, TNF-α, IFN-γ, MCP-1, RANTES (Cytometric Bead Array, CBA) and Staining of dendritic cell maturation marker CD86

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