Advanced in vitro exposure systems.

5 out of 7 Tobacco Companies have chosen VITROCELL® for research on combustion and e-cigarettes: A review of aerosol exposure systems relative to the analysis of cytotoxicity

9. Oct. 2016

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany

Poster No:STPOST26

 

David Thorne1, Roman Wieczorek2,Toshiro Fukushima3, Han-Jae Shin4, Robert Leverette5, Mark Ballantyne6, Xiang Li 7, Betsy Bombick5

1 British American Tobacco, Group R&D, Southampton, Hampshire, SO15 8TL, UK;
2 Imperial Brands PLC Company, Reemtsma Cigarettenfabriken GmbH, Albert-Einstein-Ring 7, 22761 Hamburg, Germany;
3 Japan Tobacco Inc, Scientific Product Assessment Centre, 6-2 Umegaoka, Aoba-ku Yokohama, Kanagawa 227-8512, Japan;
4 Korean Tobacco & Ginseng Corporation, 30 Gajeong-ro, Yuseong-gu, Daejeon, 305-805, Republic of Korea;
5 RAI Services Company, 401North Main Street, Winston-Salem, NC 27101, USA;
6 Covance Laboratories Ltd, Otley Road, Harrogate HG3 1PY, UK;
7 Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, No.2 Fengyang Street, High-tech Zone, Zhengzhou, PR China

 

Cytotoxicity data (NRU and MTT) from Kentucky reference 3R4F cigarette smoke were assessed on Balb/C 3T3, CHO-K1, A549, Beas-2B, NCI-H292 and HepG2 cell lines on the Vitrocell Exposure System.

Abstract

Aerosol exposure systems offer researchers a variety of ways to customize the exposure set-up, modify experimental parameters and provide a novel and versatile platform for in vitro aerosol research. These systems produce an aerosol that more closely mimics the human smoking condition with associated aerosol interactions, an advantage over the potential limitation of using aerosol fractions alone. Exposure systems typically consist of two functional parts: the smoking machine / aerosol generator, and the exposure module/multiwell plate housing the cell system. The possible combinations of exposure systems, modules and plate formats give rise to an in vitro aerosol research environment that is complex and diverse, resulting in unique combinations of variables that few laboratories share. Ultimately, this causes challenges in comparing data between set-ups using similar systems and an inability to compare data across some platforms, making tobacco aerosol research particularly difficult to contextualize across laboratories. Over recent meetings, the CORESTA In Vitro Toxicity Testing SubGroup has discussed the developing field of aerosol exposure research. Given the diversity of techniques, exposure parameters and biological end-points being deployed, it was considered a high priority to establish a strategy to assess these systems and the responses obtained. Twelve global companies with expertise in in vitro aerosol research met to discuss this topic and identify potential areas of alignment. A detailed and comprehensive survey was conducted on over 40 parameters ranging from aerosol generation, dilution, biological methodology, data analysis and dosimetry approaches, across eight independent laboratories. Survey results demonstrate the diversity of and provide awareness of the exposure systems, parameters, methodology nuances and data analysis. Results identify potential commonalities and important areas of consideration, which may be of substantial benefit to current smoke/aerosol researchers, scientists from intersecting fields of research, and new scientists and laboratories entering into this area of research.

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