Advanced in vitro exposure systems.

VITROCELL® Powder Chamber
For exposure to smallest quantities of dry powders

VITROCELL® Cloud System

1 Particle release, 2 Sedimentation tubes, 3 Exposure tray, 4 Controller

This system is specifically designed for dose-controlled and uniform deposition of dry powder aerosols on cells cultured at the air/liquid interface. The dry powder can be applied from all commercially available inhalers or via direct dosing.

A typical powder quantity for exposure is 20 mg per experiment.

Exposure Process

Duration for a single experiment lasts approx. 20 minutes. The powder is transported via negative pressure from ­the inhaler or direct dosing device to ­4 independent sedimentation tubes. After these are filled, the 4 cell culture insert compartments are moved ­under the sedimentation tubes for exposure. The entire process is sequenced automatically by ­editing the VITROCELL® Powder Chamber Controller.

Process Steps

VITROCELL® Powder Chamber Insert loading
Step 1 - Insert loading
The exposure tray houses 4 compartments for 12-well sized cell culture inserts or optional Quartz Crystal Microbalances. The tray is shown in the loading position.
VITROCELL® Powder Chamber Filling of sedimentation tubes
Step 2 - Filling of sedimentation tubes
Filling of the sedimentation tubes is performed by a controlled air flow. Subsequent sedimentation takes place via gravity and without active flow. Filling and sedimentation times are edited in the controller unit. The tray is shown in the sedimentation position.
VITROCELL® Powder Chamber Exposure to inserts
Step 3 - Exposure to inserts
After filling and sedimentation, the tray is moved to connect the sedi­mentation tubes with the cell culture insert compartments. The desired exposure duration time is edited in the controller unit. The tray is shown in the exposure position. After exposure the tray is moved back to position 1 to access the exposed inserts.

Features

  • Deposition of small particles
  • For 12-well sized inserts
  • Exposure to 4 wells
  • Controlled dry powder release
  • Deposition by sedimentation and diffusion
  • Flexible flow rates
  • Easy handling
  • Physiological air/liquid interface conditions
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